{
  "schemaVersion": 1,
  "slug": "ggassembler",
  "name": "ggassembler",
  "description": "Design a combinatorial mutation library as degenerate-codon oligos with GGAssembler — pick ambiguous codons for the positions that vary, place Golden Gate overhangs by shortest-path search, simulate the assembly, and emit the oligos to order.",
  "category": "utility",
  "version": "1.0.0",
  "author": "Heureka Labs",
  "license": "CC-BY-4.0",
  "tags": [
    "golden-gate",
    "library-design",
    "degenerate-codons",
    "protein-engineering",
    "dna-assembly"
  ],
  "covers": [
    "combinatorial library",
    "degenerate codon",
    "ambiguous codon",
    "golden gate",
    "type IIS",
    "BsaI",
    "BsmBI",
    "SapI",
    "oligo pool",
    "site-saturation mutagenesis",
    "deep mutational scanning",
    "overhang fidelity",
    "oligo order",
    "resfile"
  ],
  "papers": [
    "doi:10.1002/pro.5169",
    "doi:10.1021/acssynbio.8b00333"
  ],
  "updatedAt": "2026-10-14T08:22:56-04:00",
  "source": {
    "repo": "heurekalabsco/heurekaskills",
    "commit": "87bef344c860de2523de46617b1f9e4d0a34c5ac",
    "path": "skills/ggassembler"
  },
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  "signals": null,
  "files": [
    {
      "path": "SKILL.md",
      "url": "https://heurekaskills.com/ggassembler/files/SKILL.md",
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}